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LH Ovulation Rapid Test Cassette


LH Ovulation Rapid Test Cassette detects the human hormone LH in urine. It is used to predict the time of ovulation.

Contents Include

  • 1 test cassette

  • 1 desiccant in a sealed pouch


Storage and Stability

  • Store the sealed pouch at 4-30℃ or 39-86℉.

  • Once the pouch is opened, the test strip should be used within one hour.

  • DO NOT FREEZE the test device.

  • Avoid heat, direct sunlight, and moisture.

Specimen Collection and Handling

  • First morning urine is not recommended, but any other time of day is suitable. For best results, collect urine at a similar time of the day each day. The urine must be collected in a clean, dry container.

When to start testing

The length of the menstrual cycle is the duration from your first menstrual bleeding day to the day before the next bleeding begins. Determine the length of the menstrual cycle before the test. Please refer to the chart below to determine when you should start testing. If your cycle length varies a lot, take the average number of days from the last 3 months. If your cycle is shorter than 21 days or longer than 40 days, consult a physician. If you do not know your cycle length, you may begin the test 11 days after your first period since the average cycle length is 28 days. Perform one test each day over a 5 days period, or until the LH surge has been detected.


For example, if you have a period cycle of 28 days, and the 9th in the calendar below is the first day (day one *) of menstrual bleeding, then the 19th, or day eleven of your cycle, is the day to begin testing.


Test Procedure

  1. Remove the test cassette from the sealed pouch.

  2. Hold the dropper vertically and transfer 3 full drops of urine to the specimen well of the test cassette, and then begin timing.

  3. Wait for colored lines to appear. Read the test results at 3-5 minutes.

    • NOTE: Do not read results after 5 minutes.


Results Interpretation

  1. Positive: If the test line is equal to or darker than the control line, it indicates that you will ovulate soon. If you want to be pregnant, the best time to have intercourse is after 24 hours but before 48 hours.

  2. Negative: Only one-color line appears in the control region, or the test line appears but is lighter than the control line. This means there is no LH surge.

  3. Invalid: The result is invalid if no color line appears in the control region (C), even if a line appears in the test region (T). In any event, repeat the test. If the test still fails, stop using the lot and contact your local distributor.



  • The test works only if the instructions are followed precisely.

  • The test should not be used for birth control.

  • Some prescription drugs, such as menotropins may affect the test result. Please check with your doctor if you are unsure.

  • The test only detects the hormone surge that precedes ovulation. It can’t tell if you will actually release an egg during your cycle.

Frequently Asked Questions

Q: How accurate is the LH Ovulation Test?

A: In clinical trials, the LH Ovulation Test has been shown to be >99% accurate in detecting the LH surge.

Q: Does this test replace the basal body temperature method (BBT)?

A: The shift in basal body temperature primarily indicates that ovulation has already occurred. The LH Ovulation Test indicates that ovulation is about to occur.

Q: Does alcohol, aspirin, or any other common drug affect the test?

A: No, but some hormonal medications can interfere with test results. Drugs containing hCG or LH can affect the test results. Clomid can cause false positives if you begin testing too early in your cycles. Talk to your doctor if you have a concern.

Q: Why is first morning urine not a good sample?

A: It is because most women experience a blood LH surge in the early morning. But, it does not show up in the urine until later in the day.

Q: Can test results be interpreted after five minutes?

A: No. The test should be read at 3-5 minutes for best results. Though a positive result should not change for several days, a negative result may change to a false positive after the testing period. Therefore, do not read the result after 5 minutes and discard the test after reading the test.

Q: Today's control line is a different shade of red than yesterday's control line. Is this a concern?

A: No. Variations in the color of the control line will not affect the test result. Always compare the color of the test line to that of the control line from the same device on the day the test is performed. Do not compare bands from different devices.

Q: A pink background color and vertical streaking appeared in the result area during the testing period. Is this a concern?

A: No, as long as the control line appears within five minutes, the test is working properly.

Q: How long should I continue to perform the test?

A: Unless otherwise specified by a doctor, stop testing once the LH surge is detected. Five to ten days of testing may be needed to detect the LH surge, though additional testing may be required.

Q: If I am still uncertain when the test kit is positive, what shall I do?

A: Have intercourse when you think the test shows you are ovulating, and then continue to test your urine even though ovulation has passed. This will help you see the maximum darkness of your test line. The ovulation signal (LH surge) only lasts 48-60 hours. Therefore, your test line will eventually start to fade away if you continue testing after ovulation. Seeing this line fade will help you to know the peak darkness of your test line and give you more confidence in pinpointing your most fertile time.

Q: I have received a positive result and had intercourse during these fertile days. I have not become pregnant. What shall I do?

A: There are many factors that can affect the ability to become pregnant. Often it may be necessary to use the test kit for 3-4 months before getting pregnant. Talk to your doctor if pregnancy is not achieved after 3-4 months.

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